Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
Rev. bras. ciênc. avic
; 8(1)2006.
Article
em En
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LILACS-Express
| VETINDEX
| ID: biblio-1491100
Biblioteca responsável:
BR68.1
ABSTRACT
This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 120,000 and 11,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
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Base de dados:
VETINDEX
Idioma:
En
Revista:
Rev. bras. ciênc. avic
Ano de publicação:
2006
Tipo de documento:
Article