O inibidor de serino protease Antipaina preserva a integridade do acrossoma de espermatozoides bovino Curraleiro Pé-Duro durante a criopreservação / The inhibitor of serine protease Antipain preserve the integrity in the acrosome of sperm bovine Curraleiro Pé- Duro for cryopreservation

ABSTRACT

This study was conducted to evaluate the effect of different concentrations of inhibitor antipain serineprotease (10 g, 50 ug and 100 ug) in supplementing the dilutive of bovine semen freezing. Thirty-six ejaculatedfour Curraleiro Pé-Duro cattle were used for cryopreservation. The epifluorescence microscopy was used todetermine the acrosomal integrity and acrosome reaction rate was calculated after sperm were incubated for sixhours heparin. Spermatozoa cryopreserved in the presence of antipain significantly increased acrosomalintegrity, as they were able to complete the acrosome reaction in vitro. In conclusion, the inhibitor is antipainserine protease is able to preserve the integrity of cryopreserved sperm acrosomal cattle.