Efeito da melatonina sobre a viabilidade de espermatozóides criopreservados de ovinos / Effect of melatonin on the feasibility of sperm cryopreserved of ram

ABSTRACT

This study to evaluate the effect of melatonin supplementation to TRIS-GEMA diluter in sperm viabilityof semen ram cryopreserved. Was used seven ejaculates of six ram,which were pooled in pool, according to theexperimental groups T1 - control; T2 - 1x10-7 mol / L and T3 - 1x10-9 mol / L melatonin. Posteriorly thediluting the samples were packaged in straws of 0.25 ml and cryopreserved. After thawing at 37°C for 30seconds, the samples were analyzed for Test thermoresistance slow (TTR) and acrosomal membrane integrity.For statistical analysis we used the Duncan test (α = 0.05) for mean comparison. The results demonstrated thatto the TTR at time 0 min, T2 showed 54.28% motility, and T1obtained lower values in the times 0 and 60 minutesfor force. Supplementation of 1x10-9mol / L of melatonin improved total motility.