Obtenção de embriões clones de Veado-catingueiro (Mazama gouazoubira) pelo uso da transferência Nuclear de Células Somáticas interespecíficas (TNCSi) / Obtention of brown brocket deer (Mazama gouazoubira) embryos by interspecies Somatic Cell Nuclear Transfer (iSCNT)

ABSTRACT

Aiming to produce clone embryos from brown brocket deer (Mazama gouazoubira) and to evaluatetheir in vitro development, bovine oocytes were used as cytoplasts. Fibroblasts from brown brocket deer andbovine were used as carioplasts. After 20-22 hours of in vitro maturation the oocytes were denuded and thenenucleated. Embryos were reconstructed and then activated using ionomycin-DMAP protocol. The reconstructedembryos were cultured for seven days (38.5°C with 5% CO2, 5% O2 and 90% N2) and the differences betweeniSCNT (deer-bovine) and SCNT (bovine-bovine) development rates were compared by unpaired t test (p <0.05).No differences were observed in the cleavage (65.5% and 72.8%) and blastocyst (11.3% and 5.9%) rates toiSCNT and SCNT embryos, respectively. In conclusion, bovine oocytes may be used in brown brocket deerconservation programs through iSCNT technique.