Your browser doesn't support javascript.
loading
Molecular characterization of GSyV-1 and GLRaV-3 and prevalence of grapevine viruses in a grape-growing area
Moura, Cátia Jacira Martins de; Fajardo, Thor Vinícius Martins; Eiras, Marcelo; Silva, Fábio Nascimento da; Nickel, Osmar.
Afiliação
  • Moura, Cátia Jacira Martins de; Biological Institute. Dept of Plant Pathology. BR
  • Fajardo, Thor Vinícius Martins; Embrapa Grape & Wine. Bento Gonçalves. BR
  • Eiras, Marcelo; Biological Institute. Dept of Plant Pathology. BR
  • Silva, Fábio Nascimento da; Santa Catarina State University. Centre of Agroveterinary Sciences. Lages. BR
  • Nickel, Osmar; Embrapa Grape & Wine. Bento Gonçalves. BR
Sci. agric ; 75(1): 43-51, Jan.-Feb.2018. ilus, tab, graf
Article em En | VETINDEX | ID: biblio-1497685
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
The aims of this study were to determine the prevalence of viruses in 119 samples from 32 grapevine cultivars, collected from nine vineyards in a specific grape-growing area in southeastern Brazil, perform a partial molecular characterization of 14 isolates of Grapevine Syrah virus 1 (GSyV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) and assess the coat protein genetic variability of these viruses. The detection of viruses was implemented by real-time RT-PCR (reverse transcription polymerase chain reaction) aiming to detect seven viruses and one viroid. With the exception of the Grapevine Cabernet Sauvignon reovirus (GCSV), the viruses and viroid that were evaluated were widespread in the sampled areas, often in high prevalence and multiple infections, ranging from 15 % up to 76 %. Eight isolates of GSyV-1 and six of GLRaV-3, partially characterized by complete coat protein gene nucleotide sequencing and a variability study showed nucleotide identities ranging from 91 % to 99 % (GSyV-1) and from 98 % to 100 % (GLRaV-3) among themselves, respectively. Comparisons between conventional and real-time RT-PCR detections were implemented for GSyV-1 and GLRaV-3 infections. Analysis of genetic variability indicated molecular differences between GSyV-1 and GLRaV-3 isolates and negative selection acting on the coat protein gene of both viruses. This is the first report of GSyV-1 in commercial vineyards in Brazil. The survey revealed widespread infections of seven important pathogens in one prominent Brazilian grape-producing region implying contaminated grapevine cuttings in the spread of disease.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Sci. agric Ano de publicação: 2018 Tipo de documento: Article / Project document

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Sci. agric Ano de publicação: 2018 Tipo de documento: Article / Project document