Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin
Raymundo, Erica Calcagno; Hochman, Bernardo; Nishioka, Michele Akemi; Freitas, José Octávio Gonçalves de; Maximino, Jéssica Ruivo; Chadi, Gerson; Ferreira, Lydia Masako.
Acta cir. bras.;
29(4): 224-230, 04/2014. graf
Artigo
em Inglês
| VETINDEX
| ID: vti-10225
Resumo
To investigate the subcutaneous injection of carbon dioxide (CO2) on neuropeptides Calcitonin Gene-Related Peptide (CGRP) and Substance P (SP) secretion in rat skin. Fifty-six Wistar-EPM rats were distributed in two groups: one for CGRP analysis, the other for SP analysis. Each group was subdivided into four subgroups: control (Cont), control with needle (ContNd), CO2 injection (CO2Inj) and atmospheric air injection (AirInj) - with seven animals each. Sample analyses of partial skin were conducted by Western Blotting (WB). RESULTS: In SP group, there was a decrease in the amount of neuropeptides in subgroups CO2Inj and AirInj. Similarly, in CGRP group, there was a decrease in the amount of pro-CGRP neuropeptides (15 kDa) in subgroups CO2Inj and AirInj; Nevertheless, there was no decrease in the amount of CGRP (5 kDa) in any subgroups. Subcutaneous injection of CO2 and atmospheric air decreased the amount of Substance P and pro-Calcitonin Gene-Related Peptide (15 kDa) neuropeptides in rat skin.(AU)
Biblioteca responsável:
BR1.1
Localização: BR68.1
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