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Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis

Gonçalves-Albuquerque, Suênia da Cunha; Silva, Rômulo Pessoa e; Morais, Rayana Carla Silva de; Trajano-Silva, Lays Adrianne Mendonça; Régis-da-Silva, Carlos Gustavo; Brandão-Filho, Sinval Pinto; Palva-Cavalcanti, Milena de.
J. Venom. Anim. Toxins incl. Trop. Dis.; 20: 1-6, 2014. tab, ilus
Artigo em Inglês | VETINDEX | ID: vti-10968

Resumo

Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasites DNA during extraction may significantly impair its detection by PCR and lead to false-negative results. This study proposes a triplex PCR assay targeting the parasites DNA, an external control (pUC18) and an internal control (G3PD) for accurate diagnosis of leishmaniasis.(AU)
Biblioteca responsável: BR68.1
Localização: BR68.1