Effects of blocking av b3 integrin by a recombinant RGD disintegrin on remodeling of wound healing after induction of incisional hernia in rats
Oliveira, Claudio Ricardo de; Marqueti, Rita de Cassia; Cominetti, Marcia Regina; Douat, Estela San'Ana Vieira; Ribeira, Juliana Uema; Pontes, Carmen Lucia Salla; Borghi-Silva, Audrey; Selistre-de-Araujo, heloisa Sobreiro.
Acta cir. bras.;
30(2): 134-142, 2015.
Artigo
em Inglês
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| ID: vti-11839
Resumo
PURPOSE: To investigate the changes induced by DisBa-01 on repair of wound healing after induced incisional hernia (IH) in rats. METHODS: Thirty two male albino rats were submitted to IH and divided into four experimental groups: G1, placebo control; G2, DisBa-01-treated; G3, anti-αv β3 antibodies-treated and G4, anti-α2 antibodies-treated. Histological, biochemical and extracellular matrix remodeling analysis of abdominal wall were evaluated. RESULTS: After 14 days, 100% of the G2 did not present hernia, and the hernia ring was closed by a thin membrane. In contrast, all groups maintained incisional hernia. DisBa-01 also increased the number macrophages and fibroblasts and induced the formation of new vessels. Additionally, MMP-2 was strongly activated only in G2 (p<0.05). Anti- αv β3 -integrin antibodies produced similar results than DisBa-01 but not anti-α2 integrin blocking antibodies. CONCLUSION: DisBa-01 has an important role in the control of wound healing and the blocking of this integrin may be an interesting therapeutically strategy in incisional hernia.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
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