Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats
Acioli, Porfirio Cezar Passos; Albuquerque, Andrea de Oliveira; Guimarães, Isaac Bevilaqua de Albuquerque; Araujo, Roberto Wagner Bezerra de; Vasconcelos, Paulo Roberto Leitão; Guimarães, Sérgio Botelho.
Acta cir. bras.;
29(7): 450-456, 07/2014. graf, ilus
Artigo
em Inglês
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| ID: vti-12365
Resumo
PURPOSE: To evaluate the effects of acupuncture (Ac) and electroacupuncture (EAc) on oxidative stress and inflammation in testis torsion/detorsion (T/D) model in rats. METHODS: Thirty male Wistar rats were randomized into five groups. G1 Group (Sham) served as control. The remaining groups were submitted to spermatic cord torsion (720°) for 3 hours, followed by detorsion and reperfusion for 4 hours. Before detorsion G3, G4 and G5 rats were treated with Ac, EAc 2Hz and EAc 10 Hz, respectively, applied to acupoint Gulai (S-29) bilaterally under anesthesia for 5 minutes. Next, the testes were detorsioned and reperfused for 4 hours. Afterwards, blood samples and the right testis were collected for biochemical assays: reduced Glutathione (GSH), Malonaldehyde (MDA), Myeloperoxidase (MPO). RESULTS: EAc stimulation (2 and 10 Hz) promoted significant increase in concentrations of GSH in plasma and testis of G4-G5 rats, compared with G1. There was significant increase of tissue MDA in groups G4-G5 and plasma MDA in all groups, compared with G1. There was a significant reduction in MPO activity in groups G4-G5 compared with G1. CONCLUSION: Electroacupuncture stimulation (2 and 10 Hz) attenuates oxidative stress and inflammatory response in rats subjected to testicular torsion/detorsion. (AU)
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