Your browser doesn't support javascript.

Portal de Pesquisa da BVS Veterinária

Informação e Conhecimento para a Saúde

Home > Pesquisa > ()
Imprimir Exportar

Formato de exportação:

Exportar

Exportar:

Email
Adicionar mais destinatários

Enviar resultado
| |

Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

Silva Filho, Osmar Ferreira da; Argôlo Neto, Napoleão Martins; Carvalho, Maria Acelina Martins de; Carvalho, Yulla Klinger de; Diniz, Anaemilia das Neves; Moura, Laécio da Silva; Ambrósio, Carlos Eduardo; Monteiro, Janaína Munuera; Almeida, Hatawa Melo de; Miglino, Maria Angélica; Alves, Jacyara de Jesus Rosa Pereira; Macedo, Kássio Vieira; Rocha, Andressa Rego da; Feitosa, Matheus Levi Tajra; Alves, Flávio Ribeiro.
Acta cir. bras.; 29(8): 478-484, 08/2014. graf, ilus
Artigo em Inglês | VETINDEX | ID: vti-13048

Resumo

PURPOSE: To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS: Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS: Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS: Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.(AU)
Biblioteca responsável: BR1.1
Localização: BR68.1