In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens
Zimmermann, C. E. P; Machado, A. K; Cadoná, F. C; Jaques, J. A. S; Schlemmer, K. B; Lautert, C; Cruz, I. B. M; Zanette, R. A; Leal, D. B. R; Santurio, J. M.
R. bras. Ci. avíc.;
16(3): 307-312, July-Sept. 2014. tab, graf
Artigo
em Inglês
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| ID: vti-13134
Resumo
The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B1 (AFB1) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB1 concentrations in 5% CO2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB1 at 48 h (p 0.05) and of 10 and 20 µg/mL AFB1 at 72 h (p 0.01 and p 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p 0.001). ROS formation significantly increased at 24 h at all concentrations (p 0.001). The in-vitro results demonstrate that AFB1 is cytotoxic and causes biomolecular oxidative damage in broiler lymphocytes.(AU)
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BR68.1
Localização: BR68.1
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