Histologia, histoquímica e histometria do intestino de jacaré-do-Pantanal criado em cativeiro / Histology, histochemistry and histometry of the intestine of Cayman yacare bred in captivity

RESUMO

A criação de jacaré-do-Pantanal tem se consolidado no estado de Mato Grosso como atividade alternativa e legal para as propriedades rurais na área de ocorrência natural da espécie, coibindo a caça predatória e colaborando na preservação da espécie.(AU)

ABSTRACT

Commercial breeding of Caiman yacare has been consolidated in the state of Mato Grosso, Brazil, as an alternative and legal activity for rural properties in the area of its natural occurrence, to restrain predatory hunting and for preservation of the species. Rationalization of the production process in alligator breading is a relatively new action, which allows a better skin quality and integral use, different from the production of skins from animals in nature. Considering the importance of the intestines as being the main place of the major events related to nutrients obtainment for the body metabolism, this study aimed to characterize qualitative and quantitatively the mucosa of the small and large intestine of young C. yacare. For the characterization of the intestinal wall structure in optical microscopy and histometry of the mucosa were collected intestinal samples from 16 animals, three of which were also used for the histochemical study. The samples were obtained from five regions, four from the small intestine and one from the large intestine. They were fixed in Bouin solution and processed according to the techniques recommended for paraffin inclusion. Semiserial histological sections (about four micrometers) were obtained and the specimen was deparaffinized, hydrated, and stained with hematoxylin-eosin for the general description and histometric study, which consisted of measuring the frequency of intestinal villi, lengths of epithelium and lamina propria of the mucosa, and mucosal thickness. In the statistical analysis was applied the variance analysis and Tukey test. For the histochemistry study were used the reaction to periodic acid-Schiff, alcian blue pH 1.0 counterstained with hematoxylin and alcian blue pH 2.5 conjugated to the periodic acid-Schiff. For description of the histological structures was used the terminology available in the Histological Nomina. ..(AU)