Qualidade microbiológica de salsichas comercializadas a granel e embaladas a vácuo / Microbiological quality of sausages commercialized in bulk and vacuum packed

RESUMO

Este trabalho caracterizou-se pela investigação microbiológica em 40 amostras de salsichas comercializadas a granel e embaladas a vácuo. Foram investigados Salmonella sp em 25g, coliformes termotolerantes, Clostridium sulfito redutores e Staphylococcus coagulase positiva. Nas amostras de salsichas embaladas a vácuo não ocorreu a presença dos micro-organismos investigados. Nas amostras de salsichas comercializadas a granel, não houve crescimento de Salmonella sp. em 25g e de Clostridium sulfito redutor. Em 25% das amostras analisadas ocorreu a presença de Staphylococcus coagulase positiva, porém com valores inferiores aos preconizados pela legislação vigente que é de 3,0x10³UFC/g. Ocorreu a presença de coliformes termotolerantes em 20% das amostras de salsicha a granel, com valores compreendidos entre 9,2NMP/g e 23NMP/g. Estes valores são inferiores aos padrões legais vigentes, pois para coliformes a 45°C há uma tolerância de 103NMP/g. Para as amostras que apresentaram positividade de coliformes termotolerantes, foi investigada a presença de E. coli e a sorologia aplicada aos isolados desse estudo não confirmam a presença de E. coli enteroinvasora. Quanto a E. coli enteropatogênica clássica ocorreu 07 isolados classificados como EPEC Poli A (04 026, 02 0111 e 01 0119),02 isolados EPEC Poli B (0114 e 0125) e 04 isolados de EPEC Poli C (03 0126 e 010128). (AU)

ABSTRACT

This work was characterized by microbiological investigation in 40 samples of sausages commercializedin vacuum pack and in bulk. Were investigated Salmonella sp in 25g, thermotolerant coliforms,Clostridium sulphite reducing and positive Staphylococcus coagulase. In the samples of sausages vacuum packed the presence of microorganisms investigated were not found. In the samples of sausages commercialized in bulk, there was no growth of Salmonella sp. at 25 grams and Clostridium sulphite reducing. In 25% of the analyzed samples was found the presence of positive coagulase S. aureus, however with values lower than recommended by the legislation which is 3,Ox103UFC/g. We found thepresence of thermotolerant coliforms in 20% of samples of bulked sausages, with values found between 9,2NMP/g e 23NMP/g. These values are lower than the current legal standards, which for coliforms at 45°C there is a tolerance of 103NMP/g. For samples that had shown positiveness for thermotolerant coliforms was investigated the presence of E. coli and the serology applied to the isolated ones fromthis study do not confirm the presence of enteroinvasive E. coli. ln relation to the Classic Enteropathogenic E.coli we found 07 isolated ones that were classified as EPEC PoliA (04 026,020111 and 01 O119), 02 EPEC Poli B (O 114 and 0125) isolated ones and 04 EPEC Poli C (03 0126 and 01 0128) isolated ones. (AU)