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Characterization of mannitol-fermenting methicillin-resistant staphylococci isolated from pigs in Nigeria
Ugwu, Clifford C; Gomez-Sanz, Elena; Agbo, Ifeoma C; Torres, Carmen; Chah, Kennedy F.
Afiliação
  • Ugwu, Clifford C; Federal University of Technology. Department of Animal Science and Technology. Owerri. Nigeria
  • Gomez-Sanz, Elena; University of La Rioja. Department of Food and Agriculture. Logrono. Spain
  • Agbo, Ifeoma C; Federal University of Technology. Department of Animal Science and Technology. Owerri. Nigeria
  • Torres, Carmen; Federal University of Technology. Department of Animal Science and Technology. Owerri. Nigeria
  • Chah, Kennedy F; Federal University of Technology. Department of Animal Science and Technology. Owerri. Nigeria
Braz. J. Microbiol. ; 46(3): 885-892, July-Sept. 2015. tab, ilus
Article em En | VETINDEX | ID: vti-14826
Biblioteca responsável: BR1.1
Localização: BR68.1
ABSTRACT
This study was conducted to determine the species distribution, antimicrobial resistance pheno- and genotypes and virulence traits of mannitol-positive methicillin-resistant staphylococci (MRS) isolated from pigs in Nsukka agricultural zone, Nigeria. Twenty mannitol-positive methicillin-resistant coagulase-negative staphylococcal (MRCoNS) strains harboring the mecA gene were detected among the 64 Staphylococcus isolates from 291 pigs. A total of 4 species were identified among the MRCoNS isolates, namely, Staphylococcus sciuri (10 strains), Staphylococcus lentus (6 strains), Staphylococcus cohnii (3 strains) and Staphylococcus haemolyticus (one strain). All MRCoNS isolates were multidrug-resistant. In addition to β-lactams, the strains were resistant to fusidic acid (85%), tetracycline (75%), streptomycin (65%), ciprofloxacin (65%), and trimethoprim/sulphamethoxazole (60%). In addition to the mecA and blaZ genes, other antimicrobial resistance genes detected were tet(K), tet(M), tet(L), erm(B), erm(C), aacA-aphD, aphA3, str, dfrK, dfrG, catpC221, and catpC223. Thirteen isolates were found to be ciprofloxacin-resistant, and all harbored a Ser84Leu mutation within the QRDR of the GyrA protein, with 3 isolates showing 2 extra substitutions, Ser98Ile and Arg100Lys (one strain) and Glu88Asp and Asp96Thr (2 strains). A phylogenetic tree of the QRDR nucleotide sequences in the gyrA gene revealed a high nucleotide diversity, with several major clusters not associated with the bacterial species. Our study highlights the possibility of transfer of mecA ...(AU)
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2015 Tipo de documento: Article