Your browser doesn't support javascript.

Portal de Pesquisa da BVS Veterinária

Informação e Conhecimento para a Saúde

Home > Pesquisa > ()
Imprimir Exportar

Formato de exportação:

Exportar

Exportar:

Email
Adicionar mais destinatários

Enviar resultado
| |

Effect of L-arginine treatment on motility, hyperactivity, acrosome reaction of ejaculated ram spermatozoa

El-Shahat, K. H; Taysser, M. I; Badr, M. R; Zaki, K.
Anim. Reprod. (Online); 13(2): 75-80, abr.-jun. 2016. tab
Artigo em Inglês | VETINDEX | ID: vti-17428

Resumo

The aim of this study was to evaluate the effect of in vitro treatment of ejaculated ram spermatozoa with different concentrations of L-arginine at various incubation times on motility, hyperactivity (HA) and acrosome reaction. Freshly ejaculated spermatozoa collected from three rams were pooled and subjected to the swim up technique in modified sperm Tyrode's albumin lactate pyruvate (S-TALP) medium supplemented with different concentrations of Larginine (0.01, 0.02, 0.03,0.04 and 0.05 mM) at 30, 60,90 and 120 min of incubation. Following sperm incubation, the following parameters were examined: motility, hyperactivity (HA) and acrosome reaction (AR). The results showed that irrespective of the concentration, incubation of ram spermatozoa with Larginine for 30min did not significantly affect the motility. However, increase the time of incubation for more than 30 min significantly decreased (P < 0.05) the motility of the spermatozoa as compared to the control. The lowest motility was recorded when spermatozoa were incubated with 0.05 mM L-arginine for 120 min. Treatment of ram spermatozoa with 0.05 mM Larginine resulted in a significant (P < 0.05) increase in HA% immediately after dilution compared to the control. A significant (P < 0.05) increase in total AR% was concomitant to the increase in the concentration of L-arginine with highest AR achieved at 0.04 mM and 90 min incubation. However, increasing the time of incubation to 120 min significantly decreased (P < 0.05) the percentage of spermatozoa with total AR compared to the other incubation times at 0.02, 0.04, and 0.05 mM L-arginine. In conclusion, under our experimental conditions treatment of ejaculated ram spermatozoa with 0.04 mM of L-arginine for 90 min was considered the best concentration of L-arginine to be used for in vitro induction of acrosome reaction.(AU)
Biblioteca responsável: BR68.1
Localização: BR68.2