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Low density lipoproteins at 2% concentration can replace whole egg yolk in tes-tris-milk extender for freezing buffalo sperm cells
Brito, Mayara Ferreira; Neves, Beatriz Parzewski; Andrade, Guilherme de Oliveira; Gouvêa, Rafael Ruas; Almeida, Jaci; Morais, Camila Leite; Martins Filho, Olindo Assis; Araújo, Márcio Sobreira Silva; Melo, Maria Isabel Vaz de; Henry, Marc.
Afiliação
  • Brito, Mayara Ferreira; Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte. BR
  • Neves, Beatriz Parzewski; Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte. BR
  • Andrade, Guilherme de Oliveira; Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte. BR
  • Gouvêa, Rafael Ruas; Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte. BR
  • Almeida, Jaci; Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte. BR
  • Morais, Camila Leite; Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte. BR
  • Martins Filho, Olindo Assis; Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte. BR
  • Araújo, Márcio Sobreira Silva; Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte. BR
  • Melo, Maria Isabel Vaz de; Pontifícia Universidade Católica de Minas Gerais. Belo Horizonte. BR
  • Henry, Marc; Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte. BR
Acta sci. vet. (Impr.) ; 46: 1-6, 2018. tab, graf
Article em En | VETINDEX | ID: biblio-1457810
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

Background:

Over the years, the most commonly used extenders for semen cryopreservation contain egg yolk as cryoprotectant. However, more recent studies have used the low density lipoproteins, extract of hen egg yolk which is responsible for the cryoprotective effect. Nevertheless, little was known about its required minimum concentration as well as its interaction with other extra cellular cryoprotectants, like skimmed milk. The present study aimed at investigating the effect of replacing whole egg yolk by adding low density lipoproteins at low concentrations, in TES-Tris-skim milk based extender, on the post-thaw quality of buffalo bull sperm.Materials, Methods &

Results:

Eighteen ejaculates were collected from six buffalo bulls and diluted with TES-Tris-skim milk based extender containing LDL, extracted from hen egg yolks, at the concentrations of 2%, 4%, 8% and 14%, against a control extender containing 20% fresh egg yolk. After semen collection, analyses of subjective motility, vigor, force tourbillon, sperm concentration (Neubauer chamber) and sperm morphology (phase contrast microscopy) were performed. The diluted semen was packaged in 0.25 mL straws, and cooling was performed on computerized machine (TK 4000®), using a cooling rate of -0.25°C/min to 5°C. Semen was kept in balance at 5°C for 4 h. The straws were frozen in an ice chest, kept at 5 cm from the surface of liquid nitrogen for 20 min and then immersed in liquid nitrogen. The samples were kept in cryogenic container until thawing. Post-thaw kinetic parameters during incubation at 37°C (CASA), sperm membrane integrity (SYBR-14/PI), membrane functionality (hypo-osmotic swelling test) and DNA fragmentation (%DFI - SCSA) were evaluated after thawing. Immediately post-thaw, total motility was higher in the control (56.53 ± 9.73) than in the tested extenders; however, after 30 min the difference was no longer detected.[…]
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Acta sci. vet. (Impr.) / Acta sci. vet. (Online) Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Acta sci. vet. (Impr.) / Acta sci. vet. (Online) Ano de publicação: 2018 Tipo de documento: Article