Your browser doesn't support javascript.
loading
Confirmation and Sequence analysis of N gene of PPRV in South Xinjiang, China / Confirmação e análise de sequência do gene N de PPRV em Xinjiang do Sul, China
Liu, YongHong; Yang, Bo; Li, XianQiang; Wang, XueFeng; Zhang, LuYao; Li, Fei; Zhao, Li.
Afiliação
  • Liu, YongHong; Tarim University. College of Animal Science. Key Laboratory of Tarim Animanl Husbandry Science and Technology of Xinjiang Production & Construction Corps. Aral. China
  • Yang, Bo; Animal Disease Control Center of Ordos. Ordos. China
  • Li, XianQiang; Tarim University. College of Animal Science. Key Laboratory of Tarim Animanl Husbandry Science and Technology of Xinjiang Production & Construction Corps. Aral. China
  • Wang, XueFeng; Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology. Harbin. China
  • Zhang, LuYao; Tarim University. College of Animal Science. Key Laboratory of Tarim Animanl Husbandry Science and Technology of Xinjiang Production & Construction Corps. Aral. China
  • Li, Fei; Animal Disease Control Center of Ordos. Ordos. China
  • Zhao, Li; Tarim University. College of Animal Science. Key Laboratory of Tarim Animanl Husbandry Science and Technology of Xinjiang Production & Construction Corps. Aral. China
Arq. bras. med. vet. zootec. (Online) ; 69(5): 1105-1113, set.-out. 2017. tab, ilus
Article em En | VETINDEX | ID: vti-18095
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
In China, Peste des petits ruminants (PPR) was officially first reported in 2007. From 2010 until the outbreak of 2013, PPRV infection was not reported. In November 2013, PPRV re-emerged in Xinjiang and rapidly spread to 22 P/A/M (provinces, autonomous regions and municipalities) of China. In the study, suspected PPRV-infected sheep in a breeding farm of South Xinjiang in 2014 were diagnosed and the characteristics of complete sequence of N protein gene of PPRV was analyzed. The sheep showed PPRV-infected signs, such as fever, orinasal secretions increase, dyspnea and diarrhea, with 60% of morbidity and 21.1% of fatality rate. The macroscopic lesions after autopsy and histopathological changes were observed under light microscope including stomatitis, broncho-interstitial pneumonia, catarrhal hemorrhagic enteritis and intracytoplasmic eosinophilic inclusions in multinucleated giantcell in lung. The formalin-fixed mixed tissues samples were positive by nucleic acid extraction and RT-PCR detection. The nucleotide of N protein gene of China/XJNJ/2014 strain was extremely high homology with the China/XJYL/2013 strain, and the highest with PRADESH_95 strain from India in exotic strains. Phylogenetic analysis based on complete sequence of N protein gene of PPRV showed that the China/XJNJ/2014 strain, other strain of 2013-2014 in this study and Tibetan strains all belonged to lineage Ⅳ, but the PPRV strains of 2013-2014 in this study and Tibetan strains were in different sub-branches.(AU)
RESUMO
Na China, Peste des petits ruminants (PPR) foi relatado oficialmente em 2007. De 2010 até o surto de 2013, não houve relato de infecção por PPRV. Em Novembro de 2013, PPRV ressurgiu em Xinjiang e rapidamente se espalhou para 22 P/A/M (províncias, regiões autônomas e municípios) da China. No estudo, ovelhas com suspeita de infecção por PPRV em uma fazenda de reprodução no sul de Xinjiang form diagnosticadas em 2014 e as características da sequência completa da proteína N do gene do PPRV foi analisada. As ovelhas tinham sinais de infecção pelo PPRV, como febre, aumento de secreções oro-nasais, dispneia e diarreia, com 60% de morbidade e 21.1% de fatalidade. As lesões macroscópicas após mudanças histopatológicas foram observadas sob microscópio, incluindo estomatite, pneumonia bronco-intersticial, enterite hemorrágica catarral e inclusões eosinofílicas intracitoplasmáticas em células gigantes multinucleares no pulmão. As amostras de tecido fixadas em formalina testaram positivo para detecção de RT-PCR por extração de ácido nucleico. Os nucleotídeos da proteína N do gene da linhagem China/XJNJ/2014 apresentou extrema homologia com o China/XJYL/2013, e homologia ainda maior com a variedade PRADESH-95 da Índia. Análise filogenética baseada na sequencia completa da proteína N do gene de PPRV mostrou que as variedades China/XJNJ/2014, outra de 2013-2014 mostrada nesse estudo e as Tibetanas todas pertenciam à linhagem Ⅳ, mas as PPRV de 2013-2014 nesse estudo e as Tibetanas estavam em diferentes agrupamentos.(AU)
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Arq. bras. med. vet. zootec. (Online) Ano de publicação: 2017 Tipo de documento: Article / Project document

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Arq. bras. med. vet. zootec. (Online) Ano de publicação: 2017 Tipo de documento: Article / Project document