Platelet-derived growth factor-BB (PDGF-BB) improves follicular survival, oocyte and follicular diameters, in a dose-dependent manner, after the in vitro culture of goat preantral follicles enclosed in ovarian tissue fragments
Silva, Renato Félix da; Brito, Ivina Rocha; Lima, Laritza Ferreira de; Aguiar, Francisco Léo Nascimento de; Rodrigues, Giovanna Quintino; Nascimento, Ivila Lorrine Castro do; Rocha, Rebeca Magalhães Pedrosa; Cibin, Francielli Weber Santos; Wheeler, Matthew Bryan; Campello, Claudio Cabral; Rodrigues, Ana Paula Ribeiro; Figueiredo, José Ricardo de.
Anim. Reprod.;
14(4): 1095-1102, Oct.-Dec. 2017. ilus, tab, graf
Artigo
em Inglês
| VETINDEX
| ID: vti-18213
Resumo
The aims of this study were to investigate the effects of different concentrations of Platelet-derived growth factor-BB (PDGF-BB) on the survival, activation, levels of ROS, and growth of goat preantral follicles enclosed in ovarian tissue. For this, ovarian fragments were cultured for 7 days in Alpha Minimum Essential Medium (α-MEM+ ) with or without PDGF-BB (0, 25, 50 and 100 ng/ml). The results showed that both the 25 ng/ml PDGF and the 50 ng/ml PDGF treatments maintained the percentage of morphologically normal follicles from day 1 to day 7. In addition, the 25 ng/ml PDGF treatment showed a significantly higher percentage of morphologically normal follicles when compared to the other treatments. At day 7, greater (P < 0.05) follicular and oocyte diameters were observed in the 25 ng/ml PDGF and the 50 ng/ml PDGF treatments when compared to the cultured control treatment. On day 7 of culture, all the treatments tested had a significant increase in the percentage of developing follicles when compared to the non-cultured control. However, the percentage of follicle activation, as well as ROS production, were similar (P < 0.05) among the treatments, irrespective of culture time. In conclusion, PDGF-BB improved, in a concentration-dependent manner, follicular survival as well as oocyte and follicular diameter after in vitro culture of goat preantral follicle-enclosed in ovarian tissue fragments.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
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