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Efficacy of phosphocreatine pre-administration on XIAP and Smac in ischemic penumbra of rats with focal cerebral ischemia reperfusion injury
Wang, Wei; Wang, Qi; Yu, Wanyou; Chen, Lianhua; Li, Zhong.
Afiliação
  • Wang, Wei; Nanjing Medical University. Jiangning Hospital Affiliated. Department of Anesthesiology. Nanjing. China
  • Wang, Qi; Nanjing Medical University. Jiangning Hospital Affiliated. Department of Anesthesiology. Nanjing. China
  • Yu, Wanyou; Nanjing Medical University. Jiangning Hospital Affiliated. Department of Anesthesiology. Nanjing. China
  • Chen, Lianhua; Nanjing Medical University. First Peoples Hospital of Shanghai Affiliated. Department of Anesthesiology. Shanghai. China
  • Li, Zhong; Nanjing Medical University. School of Public Health. Key Laboratory of Modern Toxicology (Ministry of Education). Nanjing. China
Acta cir. bras. ; 33(2): 117-124, fev. 2018. tab, graf, ilus
Article em En | VETINDEX | ID: vti-18345
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

Purpose:

To observe the efficacy of phosphocreatine pre-administration (PCr-PA) on X-linked inhibitor of apoptosis protein (XIAP), the second mitochondia-derived activator of caspase (Smac) and apoptosis in the ischemic penumbra of rats with focal cerebral ischemia-reperfusion injury (CIRI).

Methods:

A total of 60 healthy male Sprague Dawley (SD) rats were randomly divided into three groups (n=20) group A (the sham operation group), group B [intraperitoneally injected with 20 mg/kg (10 mg/ml) of saline before preparing the ischemia-reperfusion (IR) model], and group C [intraperitoneally injected with 20 mg/kg (10 mg/ml) of PCr immediately before preparing the IR model]. After 24 h for reperfusion, the neurological function was evaluated and the tissue was sampled to detect expression of XIAP, Smac and caspase-3 positive cells in the ischemic penumbra so as to observe the apoptosis.

Results:

Compared with group B, neurological deficit scores, numbers of apoptotic cells, expression of Smac,caspase-9 and the numbers of Caspase-3 positive cells were decreased while expression of XIAP were increased in the ischemic penumbra of group C.

Conclusions:

Phosphocreatine pre-administration may elicit neuroprotective effects in the brain by increasing expression of X-linked inhibitor of apoptosis protein, reducing expression of second mitochondia-derived activator of caspase, and inhibiting the apoptosis in the ischemic penumbra.(AU)
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