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Effects of the application of the amniotic membrane in the healing process of skin wounds in rats
Campelo, Mariana Barbosa Dias; Santos, Joelita de Alencar Fonseca; Maia Filho, Antonio Luiz Martins; Ferreira, Daniel Cabral Leão; SantAnna, Luciana Barros; Oliveira, Rauirys Alencar de; Maia, Leonardo Fonseca; Arisawa, Emilia Ângela Loschiavo.
Afiliação
  • Campelo, Mariana Barbosa Dias; Universidade do Vale do Paraíba. São Jose dos Campos. Brasil
  • Santos, Joelita de Alencar Fonseca; Universidade do Vale do Paraíba. São Jose dos Campos. Brasil
  • Maia Filho, Antonio Luiz Martins; Universidade Estadual do Piauí. Laboratório de Biotecnologia e Biodiversidade. Teresina. Brasil
  • Ferreira, Daniel Cabral Leão; Universidade Estadual do Piauí. Laboratório de Biotecnologia e Biodiversidade. Teresina. Brasil
  • SantAnna, Luciana Barros; Universidade do Vale do Paraíba. São Jose dos Campos. Brasil
  • Oliveira, Rauirys Alencar de; Universidade Estadual do Piauí. Departamento de Fisioterapia. Teresina. Brasil
  • Maia, Leonardo Fonseca; Universidade Federal do Piauí. Departamento de Medicina. Teresina. Brasil
  • Arisawa, Emilia Ângela Loschiavo; Universidade do Vale do Paraíba. Laboratório de Bioestimulação e Reparo de Tecido. São Jose dos Campos. Brasil
Acta cir. bras. ; 33(2): 144-155, fev. 2018. ilus, graf
Article em En | VETINDEX | ID: vti-18349
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

Purpose:

To evaluate the efficacy of the application of the human amniotic membrane (HAM) on the inflammatory process, fibroblast proliferation, formation of collagenand reduction of skin wound areas in rats.

Methods:

Thirty six rats were submitted to a surgical injury induction and divided into two groups (n = 18) group C (control) and T (treated with the HAM). The macroscopic evolution in the wound area and the histological characteristics of the skin samples were evaluated.

Results:

The regression of the wound area was greater in group T. The histological analysis revealed a significant reduction (p < 0.05) in the inflammatory infiltrate in group T at all experimental periods compared with that in the control group. Furthermore, the group T presented a significant increase in the proliferation of fibroblasts at 14 and 21 days compared with group C (p < 0.05). Regarding the deposition of mature collagen fibers, there was an increase in the replacement of type III collagen by type I collagen in group T (p < 0.05).

Conclusion:

Treatment with the HAM reduced the healing time as well as the inflammatory responses, increased the proliferation of fibroblasts, and induced a higher concentration of mature collagen fibers.(AU)
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