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A Cardioprotective role of Nerium oleander with the Expression of hypoxia inducible factor 2A mRNA by increasing antioxidant enzymes in rat heart tissue
Hitit, Mustafa; Corum, Orhan; Corum, Duygu Durna; Donmez, Huseyin; Cetin, Gul; Dik, Burak; Er, Ayse.
Afiliação
  • Hitit, Mustafa; Kastamonu University. Faculty of Veterinary Medicine. Department of Animal Genetics. Kastamonu. TR
  • Corum, Orhan; Kastamonu University. Faculty of Veterinary Medicine. Department of Pharmacology and Toxicology. Kastamonu. TR
  • Corum, Duygu Durna; Kastamonu University. Faculty of Veterinary Medicine. Department of Pharmacology and Toxicology. Kastamonu. TR
  • Donmez, Huseyin; Necmettin Erbakan University. Medicine Faculty. Deparment of Medical Biology. Konya. TR
  • Cetin, Gul; Erzincan University. Faculty of Pharmacy. Department of Biochemistry. Erzincan. TR
  • Dik, Burak; Selcuk University. Faculty of Veterinary Medicine. Department of Pharmacology and Toxicology. Konya. TR
  • Er, Ayse; Selcuk University. Faculty of Veterinary Medicine. Department of Pharmacology and Toxicology. Konya. TR
Acta sci. vet. (Impr.) ; 46: 1-8, 2018. tab, graf
Article em En | VETINDEX | ID: biblio-1457872
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

Background:

Nerium oleander (NO) distillate is used to either protect heart cells against oxidative stress or reduce the risk of cardiovascular disease by regulating the production of reactive oxygen species. Hypoxia-inducible factors (HIFs) regulate cellular antioxidant defense mechanisms under hypoxic conditions in which heart cells survive; however, the key responsible mechanism of NO distillate for cardioprotection remains elusive. The objective of this study was to evaluate the effects on heart tissue at different time intervals after administering NO distillate intraperitoneally (IP) while considering the transcriptional regulation of HIFs and representative antioxidant enzymes.Materials, Methods &

Results:

The NO plant was chopped, and distillated water was added. The mixture was distilled, and the distillate separated and collected into tubes, after which it was lyophilized to obtain dry material. Twenty male Wistar albino rats (2-3 month-old, 250-300 g each) were used in the study. The rats were randomly divided into four groups. The control group (n = 5) received IP injections of saline; the remaining 15 rats received IP injections of a single dose of 7.5 mL NO distillate. The NO distillate injected rats were divided into three groups according to the time from injection to harvest the heart tissue samples. The tissues were collected at 0 h (control; n = 5), 2 h (group 2; n = 5), 4 h (group 3; n = 5), and 8 h (group 4; n = 5) after injection and under general anesthesia (60 mg/kg ketamine, IP + 10 mg/kg xylazine, IP). Quantitative polymerase chain reaction (qPCR) was used to assess the expression profiles of the genes of interest in the heart tissues. Hypoxanthine phosphoribosyltransferase was used as the reference gene.[...]
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Acta sci. vet. (Impr.) / Acta sci. vet. (Online) Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Acta sci. vet. (Impr.) / Acta sci. vet. (Online) Ano de publicação: 2018 Tipo de documento: Article