Immune response and use of organic acids in broilers challenged with Salmonella Minnesota / Resposta imunológica e uso de ácidos orgânicos em frangos desafiados com Salmonella Minnesota
Pickler, Larissa; Muniz, Eduardo Correa; Kuritza, Leandro Nagae; Lourenço, Mariana Camargo; Santin, Elizabeth.
Acta sci. vet. (Online);
42: Pub. 1203, 6 set. 2014. tab, graf
Artigo
em Português
| VETINDEX
| ID: vti-19217
Resumo
Background: Salmonella control in poultry industry is of great importance to prevent foodborne diseases in humans. Theintense control of Salmonella Enteritidis caused an increased isolation of other serotypes as Salmonella Minnesota (SM),and it cannot be affi rmed that additives used to control Salmonella Enteritidis showed the same effi cacy to control of otherserovars. Therefore, the aim of this study was to evaluate the pathogenesis of SM in challenged broilers and the use ofdifferent organic acids for SM control in broilers.Materials, Methods & Results: In the present study, three experiments were conducted. In the fi rst experiment an in vivostudy to evaluate the pathogenesis of the SM in broilers where the birds were divided into 4 treatments with 15 animalseach: T1 - animals inoculated orally with SM, T2 - animals inoculated through feed with SM, T3 - in-contact birds cagedwith SM challenged birds and T4 - negative control. At 12, 24 and 48 h after the challenge, the crop, duodenum, cecumand liver were collected to detect SM. It was also collected fragments of jejunum, ileum and cecum for histopathologicaland immunohistochemistry analysis. At the microbiological evaluation 12 h after inoculation, it has already been possibleto observe the presence of SM in the crop, duodenum and cecum of birds challenged orally and through feed. Twentyfour h after contact with the challenged birds, the in-contact group have already shown the presence of SM in crop andduodenum. It was observed a signifi cant increase of goblet cells in the jejunum 24 h post inoculation (PI) in the orallyinoculated birds, and in the ileum 48 h PI in the feed challenged birds. In immunohistochemical evaluation, 12 h PI thebirds challenged orally have already shown a signifi cant increase in the number of CD3+ cells in the jejunum and 24 h PIa signifi cant increase of these cells in the ileum and cecum. In the second experiment an in vitro study was also performedto...(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
Texto completo