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In vivo induction of hepatocellular carcinoma by diethylnitrosoamine and pharmacological intervention in Balb C mice using Bergenia ciliata extracts / Indução in vivo de carcinoma hepatocelular por dietilnitrosoamina e intervenção farmacológica em camundongos balb c usando extratos de Bergenia ciliata
Dar, K. K; Ali, S; Ejaz, M; Nasreen, S; Ashraf, N; Gillani, S. F; Shafi, N; Safeer, S; Khan, M. A; Andleeb, S; Mughal, T. A.
Afiliação
  • Dar, K. K; Beijing University. College of Life Science and Technology. Beijing. China
  • Ali, S; Government College University Lahore. Department of Zoology. Lahore. Pakistan
  • Ejaz, M; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. Pakistan
  • Nasreen, S; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. Pakistan
  • Ashraf, N; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. Pakistan
  • Gillani, S. F; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. Pakistan
  • Shafi, N; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. Pakistan
  • Safeer, S; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. Pakistan
  • Khan, M. A; Nanyang Technological University. School of Material Science and Engineering. Nanyang. Singapore
  • Andleeb, S; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. Pakistan
  • Mughal, T. A; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. Pakistan
Braz. J. Biol. ; 79(4): 629-638, nov. 2019. tab, graf
Article em En | VETINDEX | ID: vti-19412
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
Background Hepatocellular carcinoma is the most frequent primary malignancy of liver and accounts for as many as one million deaths worldwide in a year. Objectives The aim of the present study was to evaluate the anti-cancerous efficiency of Bergenia ciliata rhizome against diethylnitrosoamine induced hepatocarcinogenesis in Balb C mice. Methods One percent diethylnitrosoamine was prepared by using 99 ml of normal saline NaCl (0.9 percent) solution to which was added 1 ml of concentrated diethylnitrosoamine (DEN) solution (0.01 g/l). Extract of Bergenia ciliata was prepared by maceration technique. Mice were classified into four groups as follows Group 1 a control group (N=7) received saline solution (3.5 l/mg), group 2 (N=14) received diethylnitrosoamine (3.5 l/mg) intraperitoneally once in a week for eight consecutive weeks, group 3 (N=7) received plant extract (150 mg/kg (Body weight)) once in a week, while group 4 (N=7) was given combination of diethylnitrosoamine (3.5 l/mg) and plant extract (150 mg/kg (Body weight)). After eight weeks of DEN induction group 2 mice were divided into two subgroups containing seven mice each, subgroup 1 was sacrificed while subgroup 2 was treated with plant extract (150 mg/kg (Body weight)) once in a week for eight consecutive weeks. Results The model of DEN injected hepatocellular carcinomic (HCC) mice elicited significant decline in levels of albumin with concomitant significant elevations in tumor markers aspartate aminotransferase, alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alpha feto protein (AFP), gamma glutamyl transferase (Y-GT), 5 nucleotidase (5NT), glucose-6-phosphate dehydrogenase (G6PDH) and bilirubin. The intraperitoneal administration of B. ciliata as a protective agent, produced significant increase in albumin levels with significant decrease in the levels of tumor markers aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alpha feto protein (AFP), gamma glutamyl transferase (Y-GT), 5 nucleotidase (5NT), glucose-6-phosphate dehydrogenase (G6PDH) and bilirubin. Conclusion Bergenia ciliata has potent antioxidant activity, radical scavenging capacity and anticancerous properties. Bergenia ciliata extracts may provide a basis for development of anti-cancerous drug.(AU)
RESUMO
Antecedentes O carcinoma hepatocelular é a neoplasia primária mais frequente do fígado e é responsável por até um milhão de mortes em todo o mundo em um ano. Objetivos O objetivo do presente estudo foi avaliar a eficiência anticancerígena do rizoma de Bergenia ciliata contra a hepatocarcinogênese induzida por dietilnitrosoamina em camundongos balb c. Métodos Um por cento de dietilnitrosoamina foi preparado usando 99 ml de solução salina normal (0,9 por cento) à qual foi adicionado 1 ml de solução concentrada de dietilnitrosoamina (DEN) (0,01 g / l). O extrato de Bergenia ciliata foi preparado pela técnica de maceração. Os ratos foram classificados em quatro grupos Grupo 1 grupo controle (N = 7) recebeu solução salina (3,5 mL / mg), grupo 2 (N = 14) recebeu dietilnitrosoamina (3,5 mL / mg) por via intraperitoneal uma vez por semana para oito semanas consecutivas, o grupo 3 (N = 7) recebeu extrato vegetal (150 mg / kg (peso corporal)) uma vez por semana, enquanto o grupo 4 (N = 7) recebeu combinação de dietilnitrosoamina (3,5 l / mg) e extrato (150 mg / kg (peso corporal). Após oito semanas do grupo de indução DEN 2 ratos foram divididos em dois subgrupos contendo sete ratos cada, subgrupo 1 foi sacrificado enquanto subgrupo 2 foi tratado com extrato vegetal (150 mg / kg)) uma vez por semana durante oito semanas consecutivas. Resultados O modelo de camundongos hepatocelulares carcinômicos (CHC) injetados com DEN provocou declínio significativo nos níveis de albumina com elevações significativas concomitantes nos marcadores tumorais aspartato aminotransferase, alanina aminotransferase (ALT), lactato desidrogenase (LDH), proteína alfa feto (AFP), gama glutamiltransferase (Y-GT), 5 nucleotidase (5NT), glicose-6-fosfato ehidrogenase (G6PDH) e bilirrubina. A administração intraperitoneal de B. ciliata como agente protetor produziu um aumento significativo nos níveis de albumina com uma diminuição significativa nos níveis dos marcadores tumorais aspartato aminotransferase, alanina aminotransferase (ALT), lactato desidrogenase (LDH), proteína alfa feto (AFP), gama glutamiltransferase (Y-GT), 5 nucleotidase (5NT), glicose-6-fosfato desidrogenase (G6PDH) e bilirrubina. Conclusão Bergenia ciliata possui atividade antioxidante potente, capacidade de eliminação de radicais livres e propriedades anticancerígenas. Extratos de Bergenia ciliata podem fornecer uma base para o desenvolvimento de drogas anti-cancerígenas.(AU)
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Braz. J. Biol. Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Braz. J. Biol. Ano de publicação: 2019 Tipo de documento: Article