Does alfa lipoic acid prevent liver from methotrexate induced oxidative injury in rats?
Çakır, Turul; Baştürk, Ahmet; Polat, Cemal; Aslaner, Arif; Durgut, Himmet; Şehirli, Ahmet Õzer; Gül, Mehmet; Velioğlu Õğünç, Ayliz; Gül, Semir; Sabuncuoglu, Mehmet Zafer; Oruç, Mehmet Tahir.
Acta cir. bras.;
30(4): 247-254, Apr. 2015. ilus, tab, graf
Artigo
em Inglês
| VETINDEX
| ID: vti-22180
Resumo
PURPOSE: To determine the antioxidant and anti-inflammatory effects of alfa lipoic acid (ALA) on the liver injury induced by methotrexate (MTX) in rats.METHODS:Thirty two rats were randomly assigned into four equal groups; control, ALA, MTX and MTX with ALA groups. Liver injury was performed with a single dose of MTX (20 mg/kg) to groups 3 and 4. The ALA was administered intraperitonealy for five days in groups 2 and 4. The other rats received saline injection. At the sixth day the rats decapitated, blood and liver tissue samples were removed for TNF-, IL-1, malondialdehyde, glutathione, myeloperoxidase and sodium potassium-adenosine triphosphatase levels measurement and histological examination.RESULTS: MTX administration caused a significant decrease in tissue GSH, and tissue Na+, K+ ATPase activity and which was accompanied with significant increases in tissue MDA and MPO activity. Moreover the pro-inflammatory cytokines (TNF-, IL- ) were significantly increased in the MTX group. On the other hand, ALA treatment reversed all these biochemical indices as well as histopathological alterations induced by MTX.CONCLUSION:Alfa lipoic acid ameliorates methotrexate induced oxidative damage of liver in rats with its anti-inflammatory and antioxidant effects.(AU)
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BR68.1
Localização: BR68.1
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