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Effects of L-arginine and L-NAME on ischemia-reperfusion in rat liver
Lucas, Márcio Luís; Rhoden, Cláudia Ramos; Rhoden, Ernani Luís; Zettler, Cláudio Galeano; Mattos, Angelo Alves de.
Afiliação
  • Lucas, Márcio Luís; University of Health Sciences of Porto Alegre. Postgraduate Program in Hepatology. Porto Alegre. Brazil
  • Rhoden, Cláudia Ramos; University of Health Sciences of Porto Alegre. Department of Pharmacology. Porto Alegre. Brazil
  • Rhoden, Ernani Luís; University of Health Sciences of Porto Alegre. Department of Surgery. Porto Alegre. Brazil
  • Zettler, Cláudio Galeano; University of Health Sciences of Porto Alegre. Department of Pathology. Porto Alegre. Brazil
  • Mattos, Angelo Alves de; University of Health Sciences of Porto Alegre. Department of Gastroenterology. Porto Alegre. Brazil
Acta cir. bras. ; 30(5): 345-352, May 2015. ilus, tab, graf
Article em En | VETINDEX | ID: vti-22958
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

PURPOSE:

To evaluated the effects of L-arginine (a NO donor) and L-NAME (Nw-nitro-L-arginine methyl ester - a NOS inhibitor) on ischemia-reperfusion in rat livers.

METHODS:

One hundred fifty two male Wistar rats were divided into four groups control (simulated surgery); hepatic IR; pretreatment with L-arginine plus hepatic IR; and L-NAME plus hepatic IR. The hepatocellular damage was evaluated at the first, third and seventh days after the procedures through the alanine-aminotransferase (ALT) and aspartate-aminotransaminase (AST) levels, as well as histopathological features vascular congestion (VC); steatosis (STE); necrosis (NEC); and inflammatory infiltration (INF). The mortality rate was also evaluated.

RESULTS:

The pretreatment with L-NAME significantly worsened the AST levels after hepatic IR (p 0.05) at first day and L-arginine demonstrated an attenuating effect on ALT levels at seventh day (p 0.05). Furthermore, the administration of L-arginine was able to reduce the VC and STE in the seventh day after hepatic IR (p 0.05). The analysis of the mortality rates did not demonstrate any difference between the groups. Nevertheless, there was not effect of L-arginine and L-NAME on the mortality of the animals.

CONCLUSION:

L-arginine/NO pathway has a role in the hepatic IR because the pretreatment with L-arginine partially had attenuated the hepatocellular damage induced by hepatic IR in rats.(AU)
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