Effect of remote ischemic preconditioning in the expression of IL-6 and IL-10 in a rat model of liver ischemia-reperfusion injury
Filho, Marco Antonio Correa Guimarães; Cortez, Erika; Garcia-Souza, Érica Patrícia; Soares, Vivian de Melo; Moura, Aníbal Sanchez; Carvalho, Laís; Maya, Maria Cristina de Araujo; Pitombo, Marcos Bettini.
Acta cir. bras.;
30(7): 452-460, July 2015. ilus, graf
Artigo
em Inglês
| VETINDEX
| ID: vti-23331
Resumo
PURPOSE: To study the effect of remote ischemic preconditioning (RIPC) in ischemia-reperfusion (I/R) liver injury and in the expression of IL-6 and IL-10 in a rat model. METHODS: Thirty-six male rats were divided in three groups: Sham; I/R injury, a 45 minutes lobar liver ischemia and reperfusion; and RIPC, six cycles of four minutes of ischemia and four minutes of reperfusion on the right hindlimb followed by a 45 minutes lobar liver ischemia and reperfusion. Tissue and blood samples were collected after 1h and 3h of reperfusion for histopathological study, plasma cytokines and alanine aminotransferase (ALT) measurement. RESULTS: The histopathological study demonstrated a significant reduction in liver necrosis in the RIPC group (p 0,001). The ALT levels were also significant lower in the RIPC group (p 0.01). The cytokines assessment showed that IL-6 levels were increased in the RIPC group after 1h of reperfusion, in comparison to the I/R group (p 0.05). Interleukin-10 levels in RIPC groups did not differ significantly from I/R group. CONCLUSIONS: Remote ischemic preconditioning is effective in decreasing liver necrosis in a rat model of ischemia-reperfusion. The IL-6 expression is up-regulated and peaked at 60 min of reperfusion. There was no difference in IL-10 expression between the groups.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
Texto completo