Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
Azizoglu, Ugur; Ayvaz, Abdurrahman; Ylmaz, Semih; Karabõrklü, Salih; Temizgul, Rdvan.
Braz. J. Microbiol.;
47(3): 597-602, Jul-Set. 2016. ilus, graf
Artigo
em Inglês
| VETINDEX
| ID: vti-23386
Resumo
In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the cry1Ab into pET28a (+) expression vector. Constructed plasmids were transferred to an Escherichia coli BL21 (DE3) strain rendered competent with CaCl2. Isopropyl -D-1-thiogalactopyranoside was used to induce the expression of cry1Ab in E. coli BL21(DE3), and consequently, 130 kDa of Cry1Ab was obtained. Bioassay results indicated that recombinant Cry1Ab at a dose of 1000 µg g-1 caused 40% and 64% mortality on P. interpunctella and E. kuehniella larvae, respectively. However, the mortality rates of Bt SY49-1 strains' spore-crystal mixture at the same dose were observed to be 70% on P. interpunctella and 90% on E. kuehniella larvae. The results indicated that cry1Ab may be considered as a good candidate in transgenic crop production and as an alternative biocontrol agent in controlling stored product moths.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
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