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Effects of anti-TNF-alfa in experimental diversion colitis
Buanaim, Ronaldo Parisi; Pereira, José Aires; Campos, Fabio Guilherme; Kotze, Paulo Gustavo; Goto, Eduardo Felipe Kim; Mendonça, Roberta Laís Silva; Kanno, Danilo Toshio; Martinez, Carlos Augusto Real.
Afiliação
  • Buanaim, Ronaldo Parisi; Universidade São Francisco. Bragança Paulista. Brazil
  • Pereira, José Aires; Universidade São Francisco. Faculty of Medicine. Division of Pathology. Bragança Paulista. Brazil
  • Campos, Fabio Guilherme; Universidade de São Paulo. Faculty of Medicine. Department of Gastroenterology. Brazil
  • Kotze, Paulo Gustavo; Universidade Católica do Paraná. Cajuru University Hospital. Colorectal Surgery Unit. Curitiba. Brazil
  • Goto, Eduardo Felipe Kim; Universidade São Francisco. Faculty of Medicine. Bragança Paulista. Brazil
  • Mendonça, Roberta Laís Silva; Universidade São Francisco. Bragança Paulista. Brazil
  • Kanno, Danilo Toshio; Universidade São Francisco. Bragança Paulista. Brazil
  • Martinez, Carlos Augusto Real; Universidade São Francisco. Bragança Paulista. Brazil
Acta cir. bras. ; 34(10): e201901004, 2019. ilus, graf
Article em En | VETINDEX | ID: vti-24044
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

Purpose:

To evaluate the effects of infliximab on the inflammation of the colonic mucosa devoid from fecal stream.

Methods:

Twenty-four rats were submitted to a Hartmann's procedure. They remained for 12 weeks with the fecal derivation to development of diversion colitis on excluded colorectal stump. After this period, they were divided into 3 groups one group received intervention with saline (2.0 mL / week), other group infliximab at doses of 5 mg/kg/week and the other 10 mg/kg/week for five consecutively weeks. Concluded the intervention period, the animals were euthanized to remove colon segments with and without fecal stream. Colitis was diagnosed by histological analysis and the degree of inflammation by validated score. The neutrophilic infiltrate was evaluated by tissue expression of myeloperoxidase identified by immunohistochemical. The tissue content of myeloperoxidase was measured by computer-assisted image analysis.

Results:

The inflammatory score was high in colonic segments without fecal stream. The intervention with infliximab reduced the inflammatory score in excluded colonic segments. The content of myeloperoxidase was reduced in colonic segments of animals treated with infliximab mainly in high concentrations.

Conclusion:

Intervention with infliximab reduced the inflammation and the neutrophil infiltrate in colonic segments devoid of the fecal stream.(AU)
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