Avaliação da maturação nuclear de oócitos bovinos submetidos a três diferentes sistemas de incubação / Evaluation of nuclear maturation of bovine oocytes submitted to three different incubation systems
Campelo, Iana Sales; Teixeira, Thiago Victor Damasceno; Silva, Mirelly Mirna Alves de Sousa; Cavalcanti, Camila Muniz; Vieira, Luís Alberto; Figueiredo, José Ricardo de; Moura, Arlindo de Alencar Araripe; Freitas, Vicente José de Figueirêdo.
R. bras. Reprod. Anim.;
40(4): 215-216, Out-Dez. 2016. tab
Artigo
em Português
| VETINDEX
| ID: vti-24055
Resumo
This study aimed to evaluate three different incubation systems in nuclear maturation of bovine oocytes.Follicles of 2 to 8 mm in diameter were aspirated for obtaining cumulus-oocyte complexes (COCs). Thereafter,COCs were subjected to in vitro maturation (IVM) in TCM-199 medium supplemented at 38.5 °C for 23 h indifferent incubation systems: bench incubator with high oxygen (20% O2 in air), bench incubator with lowoxygen (5% O2) and portable incubator with low oxygen. After IVM, cumulus cells were removed and oocyteswere stained with Hoechst 33342. The slides were analyzed by a fluorescence microscope and the oocytes wereclassified into: degenerated (DG), metaphase I (MI) and metaphase II (MII). No difference (P > 0.05) wasobserved among the rates of DG, MI and MII for the different incubation systems. Therefore, the use of portableincubation system is a viable alternative for in vitro maturation of bovine oocytes.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
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