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Adição do Ácido Fólico na criopreservação de sêmen ovino da raça Santa Inês / Addition of Folic Acid in the cryopreservation of semen from Santa Ines sheep

Barros, Filipe Nunes; Sá, Priscilla Niely Costa de; Barçante, Felipe Pereira da Silva; Branco, Marlon de Araújo Castelo; Sousa Filho, Marco Antônio Celestino de; Barros, Dayse Andrade; Lopes Neto, Bruno de Oliveira; Souza, José Adalmir Torres de.
R. bras. Reprod. Anim.; 40(4): 317-319, Out-Dez. 2016. tab
Artigo em Português | VETINDEX | ID: vti-24080

Resumo

We evaluated the addition of folic acid to the extender TRIS-egg yolk used in cryopreservation, seekingimprovements for the sperm kinetics and mitochondrial activity post-thawing. Were evaluated sevenejaculates/animal from six Santa Inês by Artificial Vagina, with minimum values of motility by 70.0% and vigor3. We evaluated the seminal pool for concentration and morphology. We diluted in Tris-egg yolk extender forcryopreservation, dividing it in control, Group 2 we added 10.000 uM and Group 3 we added 5000 uM of folicacid. We froze in straws(0.25 mL). Was evaluated for motility and vigor, post-thawing by slow term-resistancetest, the times T0, T60, T120 and T180 minutes one week after. For the mitochondrial activity evaluation, wasused cationic lipophilic fluorochrome JC-1. The folic acid effects were analyzed using ANOVA followed byStudent Newman-Keuls test. The groups with folic acid showed significant higher motility. Mitochondrialactivity did not differ between groups.(AU)
Biblioteca responsável: BR68.1
Localização: BR68.1