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Angiotensina-(1-7): Efeito na maturação in vitro de oócitos ovinos / Angiotensin- (1-7): Effect on in vitro maturation of sheep oocytes

Costa, Andréia da Silva; Carvalho, Muriel Alves; Costa, Amilton Paulo Raposo; Branco, Yndyra Nayan Teixeira Carvalho Castelo; Leite, Marina Carvalho; Costa, Terysdalva Pereira da; Silva, Yanne Aciole da; Feitosa, Lauro César Soares.
R. bras. Reprod. Anim.; 40(4): 322-323, Out-Dez. 2016. tab
Artigo em Português | VETINDEX | ID: vti-24089

Resumo

Aimed to evaluate the effect of addition of angiotensin-(1-7) in the middle of in vitro maturation ofsheep oocytes. Seventy-four oocytes of sheep ovaries slaughtered in fridg were matured in vitro: I - control(n=22), II - Ang-(1-7) at 1 uM (n=24), III - Ang-(1-7) at 1 uM+A-779 at 1uM (n=28) in microdropletscontaining 100 µL of maturation medium plus 10% fetal bovine serum under mineral oil in the incubatoratmosphere 5% CO2 in air at 38.5°C for a period of 18 hours. After this period, the oocytes were denuded andobserved the extrusion of the first polar body. Statistical analysis was performed using the t test at 5%probability. The addition of angiotensin-(1-7) at 1 uM, as well as the addition of angiotensin-(1-7) at 1 uMtogether with its specific antagonist A-779 at the same concentration, decrease the in vitro maturation rate ovine oocytes.(AU)
Biblioteca responsável: BR68.1
Localização: BR68.1