Efeito da melatonina sobre a integridade das membranas plasmática e mitocondrial de espermatozoides criopreservados de ovino / Effect of Melatonin on the plasmatic and mitochondrial integrity membrane of sperm cryopreserved ram
Sá, Priscilla Niely da Costa; Branco, Yndyra Nayan Teixeira Carvalho Castelo; Rodrigues, Viviany Sousa; Nascimento, Isolda Márcia Rocha do; Barros, Filipe Nunes; Branco, Marlon de Araújo Castelo; Barçante, Felipe Pereira da Silva; Souza, Jose Adalmir Torres de.
R. bras. Reprod. Anim.;
40(4): 384-385, Out-Dez. 2016. tab
Artigo
em Português
| VETINDEX
| ID: vti-24121
Resumo
This study evaluate the effect of melatonin supplementation to TRIS-GEMA diluter in plasmatic andmitochondria membrane integrity of semen ram cryopreserved. Was used seven ejaculates of six ram,whichwere pooled in pool, according to the experimental groups: Control; T1 - 1x10-7 mol / L and T2 - 1x10-9 mol / Lmelatonin. Posteriorly the diluting the samples were packaged in straws of 0.25 ml and cryopreserved. Afterthawing at 37°C for 30 seconds, the samples were analyzed for Test thermoresistance slow (TTR) andacrosomal membrane integrity. For statistical analysis we used the Duncan test (α = 0.05) for meancomparison. Treatments 1 and 2 differed statistically control for plasma membrane integrity at times 60, 120and 180min. It was concluded that supplementation 1x10-7mol / L and 1x10-9mol / L of melatonin increased theintegrity of the plasma membrane to 60,120 and 180 minutes.(AU)
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BR68.1
Localização: BR68.1
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