Efeito da Insulina-N sobre a Motilidade Espermática em Sêmen Fresco de Ovino durante 60 minutos de armazenamento utilizado Gema de Ovo de Codorna / Insulin- N Effects on Sperm Motility in Ovine Fresh Semen over 60 minutes using Quail Egg of Yolk

ABSTRACT

The objective of this study was to evaluate the effect of n-type insulin on sheep semen on the time andtemperature motility after 60 minutes of incubation at 45ºC. To therefore 7 sheep were used, the collects weremade with the aid of the Electro ejaculator. The citrate-yolk diluent was used to dilute the ejaculates (25x106sptz/ ml) using quail egg yolk. Each ejaculate was divided into four equal fractions, corresponding to thetreatments control group (Gcont), using only tris-gem; Group 50 (G500) Diluent plus 50 IU / ml N-type Insulin;Group 100 (G100) diluent plus 100 IU / ml Insulin type N; Group 200 (G200) diluent plus 200 UI / ml N-typeInsulin. After dilution, the semen remained in a water bath at 45°C for the fast thermoresistance test on the timeevaluation (0, 20, 40, 60 Minutes), for sperm motility and vigor. The data, were submitted to the t test, and thosethat did not present parameters with normal distribution were compared by the Friedman test. According to theobtained data, the insulin in the concentrations used experimentally, it was not possible to verify positive effectof the supplementation of the same, and high concentrations decrease the sperm viability.(AU)