Produção in vitro de embriões bovinos em meio suplementado com captopril / In vitro production of embryos Cattle in medium supplemented with captopril

ABSTRACT

The aim of this study was to evaluate the effects of supplementation of maturation medium withcaptopril, and its consequences in the bovine maturation, fertilization and embryo development in vitro. 326bovine ovaries from a local slaughterhouse were used. 1101 CCOs were recovered and distributed in fourgroups G1 (n = 112), that was control group; G2 (n = 322) 20μM of captopril; G3 (n = 367) 40μM ofcaptopril; and G4 (n = 300) captopril 80μM and later submitted to IVM. The matured CCOs were fertilized, andco-incubated. After fertilization, 676 presumptive zygotes were cultured and maintained in the greenhouse for 7days. The total number of viable embryos was 12; 39; 32 and 31, respectively in the experimental groups.Considering the experimental conditions adopted, it was concluded that the addition of the Captopril in mediumof oocytes IVM positively doesnt influence embryonic development, as evidenced by the similar percentages ofembryo production.(AU)