Screening of Salmonella enteritidis specific O and H antiserum potency against isolated field strains of Salmonella from Aegean region

ABSTRACT

Background: The increasing prevalence of foodborne human salmonellosis caused by Salmonella Enteritidis is a majorfoodborne illness throughout the world. Prevention from Salmonella infection is more important in an effective ongoingscreening program. The slide agglutination test could be more widely used in developing countries to obtain local andregional data due to its rapid, valid, and relatively cheap and ease application. The main purpose of this study is to characterize Salmonella Enteritidis O and H specific polyclonal antibodies for the detection of Salmonella Enteritidis in fieldsamples by comparing with different species of Salmonella field samples isolated from Aegean Region.Materials, Methods & Results: The polyclonal antibodies used in this study were produced in rabbits as a diagnostic toolin our previous study. A total of 70 Salmonella field samples isolates collected between the years 2009 and 2012 fromthe layers and broilers poultry farms in Aegean Region were examined against Salmonella Enteritidis O and H specificpolyclonal antibodies by slide agglutination test. Isolated Salmonella suspect colonies were subjected to biochemicalidentification by VITEK 2 compact microbial identification system and further serotyping to identify the serovar wasdone in the Salmonella National Reference Laboratory of Turkey. Seventy isolates of Salmonella, representing 8 differentserotypes were obtained from field samples. In serotyping, the strains together with Salmonella Schwarzengrund, Salmonella Enteritidis, Salmonella Gallinarum, Salmonella Mbandaka, Salmonella Infantis, Salmonella Kentucky, SalmonellaVirchow and Salmonella Corvallis were determined in accordance with the Kauffman White Le Minor scheme. The resultsof Salmonella Enteritidis O and H hyperimmune rabbit sera reactivity with a variety of field strains of Salmonella by slideagglutination test demonstrated that the polyclonal...(AU)