Bacteria isolated from abscesses of small ruminants inspected in the semiarid region of Brazil / Bactérias isoladas de abscessos em pequenos ruminantes inspecionados na região semiárida do Brasil

ABSTRACT

Loss in the supply chain of small ruminants owing to condemnations of carcasses in the abattoirs and slaughterhouses is common in northeastern Brazil. This study aims to identify bacterial agents, including Mycobacterium spp., in the abscesses found in the postmortem analysis of the carcasses of sheep and goats bred in northeastern Brazil. Our analysis involved 679 goats and 1,838 sheep carcasses. Abscess samples were extracted and inoculated on blood agar and Lowenstein Jensen with pyruvate or glycerol for bacterial isolation. We then performed polymerase chain reaction of the hps 65 gene; samples positive for Mycobacterium spp. were subjected to DNA sequencing. Relative frequencies of abscesses in goats and sheep were 5.44 and 3.26%, respectively. Microbiological analysis revealed 87.7% bacterial growth in the inoculated samples. Among these, Corynebacterium pseudotuberculosis represented 67.7% of the isolates. We observed 1.9% mycobacteria growth in the abscess samples inoculated on Lowenstein-Jensen medium. PCR of DNA extracted from abscesses samples showed amplification of 0.9% of samples. After sequencing, Mycobacterium spp. isolate was identified as M. novocastrense. C. pseudotuberculosis was the main agent responsible for the formation of abscesses in the examined animals, and we did not identify any species of the M. tuberculosis complex in the examined small ruminants...(AU)

RESUMO

Perdas na cadeia produtiva dos pequenos ruminantes pela condenação de carcaças e vísceras são comuns nos matadouros/frigoríficos da região nordeste do Brasil. Este estudo objetivou identificar agentes bacterianos, inclusive Mycobacterium spp., no conteúdo de abscessos encontrados no exame post mortem de caprinos e ovinos criados em regiões do nordeste do Brasil. Foram inspecionados no exame post mortem 679 caprinos e 1.838 ovinos. Para o isolamento bacteriano as amostras foram cultivadas em meio Agar Sangue e Lowenstein Jensen contendo piruvato ou glicerol. Além do cultivo, foi realizada identificação molecular para Mycobacterium spp. pela técnica de Reação em Cadeia de Polimerase (PCR) em que foram utilizados primers que amplificam fragmentos de parte do gene hsp65. As amostras positivas para micobactérias foram submetidas ao sequenciamento de DNA. As frequências relativas de abscessos em caprinos e ovinos foram de 5,44% (37/679) e 3,26% (60/1.838), respectivamente. No exame microbiológico, utilizando o meio Ágar Sague, observou-se crescimento bacteriano em 87,7% (93/106) das amostras cultivadas. Destes, o Corynebacterium pseudotuberculosis representou 67,7% (63/93) dos isolados. Do cultivo em meio Lowenstein Jensen foram isolados micobactérias em 1,9% (2/106) das amostras de abscessos e, na PCR com DNA extraído direto das amostras de abscessos, observou-se amplificação em...(AU)