Bioquimico plasmático de cães: efeitos dos diferentes anticoagulantes em comparação com o soro / Plasmatic biochemical of dogs: effects of different anticoagulants compared to serum

RESUMO

Na rotina clínica veterinária são utilizados diferentes tipos de anticoagulantes, escolhidos de acordo com o exame a ser solicitado. Animais agitados, agressivos, de difícil contenção e de pequeno porte podem ser fatores limitantes quando se deseja aliquotar a amostra em tubos com diferentes anticoagulantes. Assim, esse trabalho teve como objetivo avaliar os efeitos dos anticoagulantes EDTA, Citrato de Sódio e Fluoreto associado ao EDTA, nos resultados dos exames bioquímicos de rotina realizados em cães e compará-los com o soro. Verificou-se que o plasma obtido com o anticoagulante EDTA resultou em alterações nas dosagens de glicose, colesterol e cálcio. Amostras obtidas com Citrato de Sódio tiveram resultados inferiores ao do soro em todos os parâmetros avaliados, com exceção da ALT. O anticoagulante Fluoreto associado ao EDTA, apenas nas dosagens de uréia e glicose, não resultou em alterações estatísticas quando comparado ao soro.

ABSTRACT

Different types of anticoagulants are used in the routine of a veterinary clinic, which are chosen based on the exam to be performed. Agitated, aggressive, of hard contention and small size animals can be a limiting factor when it is wished to separate samples into tubes with different anticoagulants. Therefore, this study had as the objective evaluate the effects of anticoagulants EDTA, sodium citrate and sodium fluorite associated with EDTA, in the results of routine plasma biochemistry performed in dogs and compare it with serum. Blood samples of 10 clinically healthy dogs were collected and placed in different tubes with each anticoagulant and one without to obtain serum. Albumin, alanine aminotransferase (ALT), calcium, total cholesterol, creatine, phosphorus, glucose, total protein (PT) and urea concentrations were measured by spectophotometric. It was found that plasma obtained with EDTA anticoagulant resulted in dosage alterations for glucose, cholesterol and calcium. Samples obtained with sodium citrate presented results lower than serum in all analyzed parameters, except for ALT. The anticoagulant sodium fluorite with EDTA only didn’t result in statistical differences when compared to serum in the parameters of urea and glucose. Thus, serum is the most trustworthy sample to determine the biochemical profile of dogs, however some biochemical’s can be performed with plasma with EDTA such as creatine, ALT, urea, albumin, PT and phosphorus.