Constitutive overexpression of GmDof17-1, a putative DOF transcription factor from soybean causing growth inhibition in tobacco
Yu, Jingjing; Shi, Guixia; Yu, Deyue.
Sci. Agric.;
71(1): 44-51, Jan-Fev. 2014. tab, ilus, graf
Artigo
em Inglês
| VETINDEX
| ID: vti-27834
Resumo
The Dof proteins belong to a large family of plant transcription factors that share a single highly conserved zinc finger and play an important role in many physiological processes. To elucidate the function of Dof in soybean, GmDof17-1 was cloned from soybean (Glycine max (L.) Merr); the open reading frame (ORF) of GmDof17-1 is 846 bp and encodes a putative protein that includes 281 amino acids. Using qRT-PCR, the expression profiles of GmDof17-1 were obtained for various parts of the soybean plant. GmDof17-1 was primarily expressed in the roots and pods at various stages of pod development. The gene was ectopically expressed in tobacco under the control of the 2 × CaMV35S promoter to study the functions of the gene product. The transgenic tobacco plants showed to be dwarf, and the indole-3-acetic acid (IAA) content was decreased, whereas the sulfur-containing amino acid content of the seeds increased. These results provide new insight into the function of GmDof17-1 in seed development.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
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