Histological analysis of the intestinal wall of newborn rats submitted to hypoxia and reoxygenation to evaluate the protective effect of N-Acetylcysteine
Silvares, Soraia Guerra; Moron, Antonio Fernandes; Simões, Manuel de Jesus; Cintra, Álvaro Ulhoa; Montero, Edna Frasson de Souza; Araujo Júnior, Edward; Martins, José Luiz.
Acta cir. bras.;
35(4): e202000401, June 5, 2020. ilus, tab
Artigo
em Inglês
| VETINDEX
| ID: vti-28077
Resumo
Purpose To evaluate the effect of N-Acetylcysteine (NAC) in newborn rats submitted to hypoxia and reoxygenation (H/R) conditions in an experimental model of necrotizing enterocolitis. Methods Eight pregnant rats and their 70 cubs were used (5 groups) and exposed to H/R conditions and received NAC at different times. The animals in the H/R groups were placed in a gas chamber (100% CO2) for 10 minutes and then reoxygenated for 10 minutes (100% O2), twice a day for the first three days of life, with a six-hour span between events. On the third day of life, the animals were anesthetized, laparotomized and the intestines were resected. Results The H/R and NAC groups showed changes in the intestinal wall in relation to the number, height and width of the villi when compared to the control group (p 0.0001), but with better preservation of structures in the NAC group. There were no differences between groups regarding the number (%) of mitoses. Conclusion The administration of NAC decreased the lesions in the intestinal wall of rats submitted to H/R, therefore suggesting that this drug can be used to prevent the development of necrotizing enterocolitis in newborns.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
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