Lack of association among TNF-alfa gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori
Zabaglia, Luanna Munhoz; Ferraz, Mariane Avante; Pereira, Weendelly Nayara; Orcini, Wilson Aparecido; Labio, Roger Willian de; Caleman Neto, Agostinho; Wisnieski, Fernanda; Oliveira, Juliana Garcia de; Smith, Marilia de Arruda Cardoso; Payão, Spencer Luiz Marques; Rasmussen, Lucas Trevizani.
J. Venom. Anim. Toxins incl. Trop. Dis.;
21: 1-7, Dec. 31, 2015. tab
Artigo
em Inglês
| VETINDEX
| ID: vti-29149
Resumo
Background Tumor necrosis factor plays a critical role in the pathogenesis of gastric diseases such as gastric cancer, and an abnormal inflammatory response has frequently been observed in dyspeptic patients. Helicobacter pylori infection can induce a gastric mucosal inflammatory response that may be influenced by -308 (G > A) polymorphisms and gene expression of theTNF- gene. Methods One hundred and thirty-four gastric biopsy samples were collected from patients of both genders (61 and 73, mean age 40.3 ± 24.2 years) with gastric symptoms. The -308 (G > A) polymorphism of TNF- was characterized using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The expression level was measured using real-time PCR, and relative quantification (RQ) was calculated using the comparative CT method (2-CT). Results The analysis revealed an increase in TNF- gene expression in patients with gastritis; on the other hand, no statistical differences were observed in patients with gastric cancer. In addition, no association was found among -308 polymorphism genotypes, virulence markers, or TNF- gene expression. Conclusions Helicobacter pylori induces a large increase in TNF- expression in patients with gastritis, regardless of tissue inflammation, but after the tissue becomes neoplastic, the presence of bacteria did not influence expression. These results suggest that the TNF- pathway may play an important role in the progression from gastritis to gastric cancer.(AU)
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BR68.1
Localização: BR68.1
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