Your browser doesn't support javascript.
loading
Characterization of porcine oocytes stained with Lissamine Green B and their developmental potential in vitro
Bartkova, Alexandra; Morovic, Martin; Strejcek, Frantisek; Murin, Matej; Benc, Michal; Percinic, Florina Popovska; Laurincik, Jozef.
Afiliação
  • Bartkova, Alexandra; Constantine the Philosopher University in Nitra. Faculty of Natural Sciences. Nitra. SK
  • Morovic, Martin; Constantine the Philosopher University in Nitra. Faculty of Natural Sciences. Nitra. SK
  • Strejcek, Frantisek; Constantine the Philosopher University in Nitra. Faculty of Natural Sciences. Nitra. SK
  • Murin, Matej; Czech Academy of Sciences. Institute of Animal Physiology and Genetics. Libechov. SK
  • Benc, Michal; Constantine the Philosopher University in Nitra. Faculty of Natural Sciences. Nitra. SK
  • Percinic, Florina Popovska; Ss. Cyril and Methodius University in Skopje. Faculty of Veterinary Medicine. Skopje. MK
  • Laurincik, Jozef; Constantine the Philosopher University in Nitra. Faculty of Natural Sciences. Nitra. SK
Anim. Reprod. (Online) ; 17(4): e20200533, 2020. ilus, graf, tab
Article em En | VETINDEX | ID: biblio-1461537
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
Traditional methods for the evaluation of oocyte quality are based on morphological classification of the follicle, cumulus-oocyte complex, polar body and meiotic spindle. This study is focused on the differences between the morphological assessment of oocyte quality, the assessment based on Lissamine Green B (LB) staining and the analysis of oocytes using a proteomic approach. We evaluated the effectiveness of electrochemical and chemical parthenogenetic activation under our laboratory conditions and evaluated the applicability of Lissamine Green B staining of cumulus-oocyte complexes (COCs) as a non-invasive method for predicting the maturational and developmental competence of porcine oocytes cultured in vitro. We determined that chemical parthenogenetic activation using ionomycin and 6-dimethylaminopurine was slightly more effective than electrochemical activation. After oocyte selection according to LB staining, we found significant differences (P 0.05) between the LB- group and LB+ group and the control group in their maturation, cleavage rate and rate of blastocysts. Proteomic analyses identified a selection of proteins that were differentially expressed in each group of analysed oocytes. Oocytes of the LB- group exhibited an increased variability of proteins involved in transcription regulation, proteosynthesis and the protein folding crucial for oocyte maturation and further embryonic development. These results found a better competence of LB- oocytes in maturation, cleavage and ability to reach the blastocyst stage.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Anim. Reprod. / Anim. Reprod. (Online) Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Anim. Reprod. / Anim. Reprod. (Online) Ano de publicação: 2020 Tipo de documento: Article