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Localization and expression of ADAM2 in the dromedary camel testis, epididymis and sperm during rutting season

Al-Shabebi, Abdulkarem; Althnaian, Thnaian; Alkhodair, Khalid.
Anim. Reprod.; 18(1): e20200241, 2021. tab, ilus, graf
Artigo em Inglês | VETINDEX | ID: vti-30414

Resumo

ADAM2 (fertilin ) is a sperm surface protein reported in several mammalian species. However, the presence of ADAM2 in the male reproductive system and sperm of the camel is not well known. The present study was to clarify the localization and expression of ADAM2 in the dromedary camel testis, epididymis and spermatozoa during rutting season using immunohistochemistry (IHC) and the quantitative real-time polymerase chain reaction (qPCR). Tissue samples were obtained from the testis (proximal and distal) and epididymis (caput, corpus, and cauda) from eight mature male camels. Epididymal and ejaculated sperms were collected from four other fertile camels. IHC analysis clearly showed the localization of ADAM2 protein in the spermatocytes and the round and elongated spermatids of the testis, in the epithelial cells along the epididymis tract, on the posterior head of the sperm within the cauda epididymis, and on the acrosomal cap of both the epididymal and ejaculated sperm. The expression of camel ADAM2 mRNA was significantly higher (P 0.05) in the testis when compared with the epididymis. These findings may suggest an important role of ADAM2 in the fertility of male dromedary camels.(AU)
Biblioteca responsável: BR68.1
Localização: BR68.1