Effect of Corynebacterium cutis lysate on serum oxidative stress and plasma prostaglandin F2 alfa metabolite levels
Er, Ayse; Dik, Burak; Corum, Orhan.
Acta sci. vet. (Online);
42: Pub. 1209, Sept. 22. tab
Artigo
em Inglês
| VETINDEX
| ID: vti-30622
Resumo
Background: The Corynebacterium cutis lysate is commercial product. Unbalance between oxidants and antioxidantscause oxidative stress and lipid peroxidation in the cell. Macrophages phagocytose large pieces of bacteria and synthesizecytokines. In addition to the benefi cial results of the drug have side effects. Since changes in biochemical parameters refl ect structural dysfunction in the organism, monitoring changes of these parameters is a way to keep track of side effects.The aim of this study was to determine the effect of Corynebacterium cutis lysate on serum thiobarbituric acid-reactivesubstances (TBARS) and plasma 13,14-dihydro-15-keto-prostaglandinF2α (PGM) levels in sheep.Materials, Methods & Results: Six Merino crossbred ewes (aged >2 years, weight 40-60 kg) were used in this study. Theprocedures were approved by the Ethics Committee. A dose of 8 mg (0.4 mL) of commercial Corynebacterium cutis lysatewas subcutaneously injected to each of the 6 Merino crossbred ewes. Blood specimens were taken from the sheep prior toinjection (day 0, control) and after the injection on days 1, 2, 3, and 4. The levels of serum TBARS and plasma PGM weredetermined using an Enzyme Linked Immunosorbent Assay (ELISA) reader. The values of the hemogram [white bloodcells (WBC), red blood cells (RBC), platelets (PLT), hematocrit (HTC), and hemoglobin (HBG)] were assessed using ablood cell count apparatus. The levels of plasma creatine kinase-MB (CK-MB), serum alkaline phosphatase (ALP), alanineaminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyltransferase (GGT), total protein (TP), albumin(ALB), blood urea nitrogen (BUN), creatinine, and cholesterol were determined on an autoanalyzer. The data obtainedwere analyzed using ANOVA and Scheffes test as a post hoc test (SPSS 19.0). A P < 0.05 value was taken as the cut-offvalue for...(AU)
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