Uso de gema de ovo em diferentes concentrações na congelação espermática de Prochilodus brevis e sua influência em parâmetros cinéticos / Use of egg yolk on Prochilodus brevis sperm freezing and its influence on kinect parameters
TORRES, Thais Maia; ALMEIDA-MONTEIRO, Priscila Silva de; NASCIMENTO, Renata Vieira do; PEREIRA, Vanessa Alves; FERREIRA, Yasmim Maia; PINHEIRO, Rômulo Roberto Ribeiro; SALMITO-VANDERLEY, Carminda Sandra Brito.
Ci. Anim.;
30(04, Supl. 2): 337-341, 2020. tab
Artigo
em Português
| VETINDEX
| ID: vti-32193
Resumo
Prochilodus brevis is a fish species with an important ecological and economic role. Therefore, its captive breeding, together with sperm cryopreservation, come as an alternative to improve its availability. To perform sperm cryopreservation with quality results, it is necessary to develop studies on cryomedia composition, such as non-permeable cryoprotectants. The aim of the study was to test the efficacy of two non-permeable cryoprotectants on post-thawed kinetic parameters of Prochilodus brevis sperm. Twenty reproductive mature P. brevis males were selected and induced to sperm with carp pituitary extract. The sperm was collected, and five pools were made. Pools were diluted in 5% glucose and 10% DMSO added or not with three different concentrations of egg yolk (5; 10 or 12%). The samples were placed on French straws, frozen in dry shipper and stored in liquid nitrogen. After thawing, the kinetic analyses of computer assisted sperm analysis (CASA) were performed. There were no significant differences between the total motility of the control (60.48%) and treatments (49.0; 47.8 and 51.68%, respectively). The other kinetic parameters also showed no statistical difference. Thus, there is no need to use egg yolk in the freezing of P. brevis sperm when using a glucose and DMSO extender.(AU)
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BR68.1
Localização: BR68.1
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