Taxas de maturação de oócitos congelados lentamente de caprinos / Slowly frozen caprine oocyte maturation rate
OLIVEIRA, Marlene Sipaúba de; BRAGA, Clarissa de Castro e; TEIXEIRA, Letícia Soares de Araújo; FURTADO, Leonardo Lopes; CARDOSO, Janaína de Fátima Saraiva; MINEIRO, Ana Lys Bezerra Barradas; PAULA, Ney Rômulo de Oliveira.
Ci. Anim.;
30(04, Supl. 2): 302-306, 2020. tab
Artigo
em Português
| VETINDEX
| ID: vti-32402
Resumo
The objective was to evaluate the maturation rates of goat oocytes submitted to slow freezing in conventional medium for IVM. For this purpose, cumulus-oocyte complexes aspirated from pubic goat ovaries were classified morphologically and slowly frozen with 1.5 M ethylene glycol. After thawing the cryopreserved oocytes, those classified as viable were matured in conventional in vitro maturation medium. Evaluating the maturation rate, the percentage of matured oocytes in the group that underwent the cryopreservation process is significantly lower (17.6%) when compared to the control group (69.2%), also showing a high percentage of immature oocytes. Several oocyte injuries were found, caused by the studied cryopreservation method, interfering with their oocyte competence. Even with nuclear maturation rates, observed through the extrusion of the first polar corpuscle, the morphologies were altered in most oocytes, and further studies using new techniques and / or other cryoprotectants are necessary.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
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