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Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom
Costal-Oliveira, Fernanda; Guerra-Duarte, Clara; Oliveira, Maira Souza; Castro, Karen Larissa Pereira de; Lopes-de-Sousa, Leticia; Lara, Aline; Gomes, Enéas Ricardo de Morais; Bonilla, Cesar; Guatimosim, Sílvia; Melo, Marília Martins; Chávez-Olórtegui, Carlos.
Afiliação
  • Costal-Oliveira, Fernanda; Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte. Brasil
  • Guerra-Duarte, Clara; Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte. Brasil
  • Oliveira, Maira Souza; Federal University of Minas Gerais. College of Veterinary Medicine. Belo Horizonte. Brasil
  • Castro, Karen Larissa Pereira de; Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte. Brasil
  • Lopes-de-Sousa, Leticia; Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte. Brasil
  • Lara, Aline; Federal University of Minas Gerais. Institute of Biological Sciences. Department of Physiology and Biophysics. Belo Horizonte. Brasil
  • Gomes, Enéas Ricardo de Morais; Federal University of Minas Gerais. Institute of Biological Sciences. Department of Physiology and Biophysics. Belo Horizonte. Brasil
  • Bonilla, Cesar; Universidad Nacional Mayor de San Marcos y Peru. Instituto Nacional de Salud. Lima. Peru
  • Guatimosim, Sílvia; Federal University of Minas Gerais. Institute of Biological Sciences. Department of Physiology and Biophysics. Belo Horizonte. Brasil
  • Melo, Marília Martins; Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte. Brasil
  • Chávez-Olórtegui, Carlos; Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte. Brasil
Article em En | VETINDEX | ID: vti-32607
Biblioteca responsável: BR1.1
Localização: BR68.1
ABSTRACT
Background Hadruroides lunatus is the most abundant scorpion species in the Peruvian central coast, where most of the accidents involving humans are registered. In spite of its prevalence, there are only very few studies on H. lunatus envenomation. The aim of the present study was to analyze the cardiorespiratory alterations caused by H. lunatus envenomation in rodents. Methods Wistar rats injected with H. lunatus scorpion venom were submitted to electrocardiography. After euthanasia, rat lungs were collected and histopathologically analyzed. Mouse cardiomyocytes were used to perform immunofluorescence and calcium transient assays. Data were analyzed by ANOVA or Students t-test. The significance level was set at p< 0.05. Results It was observed that H. lunatus venom increased heart rate and caused arrhythmia, thereby impairing the heart functioning. Lungs of envenomed animals showed significant alterations, such as diffuse hemorrhage. In addition, immunofluorescence showed that H. lunatus venom was capable of binding to cardiomyocytes. Furthermore, mouse ventricular cardiomyocytes incubated with H. lunatus venom showed a significant decrease in calcium transient, confirming that H. lunatus venom exerts a toxic effect on heart. Conclusion Our results showed that H. lunatus venom is capable of inducing cardiorespiratory alterations, a typical systemic effect of scorpionism, stressing the importance of medical monitoring in envenomation cases.(AU)
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: J. Venom. Anim. Toxins incl. Trop. Dis. Ano de publicação: 2017 Tipo de documento: Article / Project document

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: J. Venom. Anim. Toxins incl. Trop. Dis. Ano de publicação: 2017 Tipo de documento: Article / Project document