Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
Ghorbanpur, M; Mirakabadi, Abbas Zare; Zokaee, F; Zolfagarrian, H.
J. Venom. Anim. Toxins incl. Trop. Dis.;
16(1): 96-106, 2010. graf, tab, ilus
Artigo
em Inglês
| VETINDEX
| ID: vti-4230
Resumo
An anticoagulant factor was purified from the venom of the Iranian snake Agkistrodon halys by gel filtration on Sephadex G-50 and ion-exchange chromatography on DEAE-Sepharose. In the final stage of purification, the percentage recovery of purified anticoagulant factor was found to be 83 percent. The purified anticoagulant factor revealed a single protein band in SDS-polyacrylamide electrophoresis under reducing conditions and its molecular weight was about 22 kDa. The purified peptide did not show any effect on casein, BApNA or plasma.(AU)
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