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A glycoprotein with anti-inflammatory properties secreted by an Aspergillus nidulans modified strain
C. F. Queiroz, J.; F. P. Teixeira, C.; Lebrun, I.; J. Spencer, P.; M. A. D. Zucchi, T..
Afiliação
  • C. F. Queiroz, J.; University of São Paulo Institute of Biomedical Sciences Department of Parasitology and Biotechnology Research Center.
  • F. P. Teixeira, C.; Butantan Institute Laboratory of Pharmacology.
  • Lebrun, I.; Butantan Institute Biochemistry and Biophysics Laboratorty.
  • J. Spencer, P.; Nuclear Energy Research Institute IVBiotechnology Center.
  • M. A. D. Zucchi, T.; University of São Paulo Institute of Biomedical Sciences Department of Parasitology and Biotechnology Research Center.
Article em En | VETINDEX | ID: vti-443165
Biblioteca responsável: BR68.1
ABSTRACT
Total RNA from lipopolysaccharide (LPS)-stimulated rat macrophages used to treat protoplasts from an Aspergillus nidulans strain originated the RT2 regenerated strain, whose culture supernatant showed anti-inflammatory activity in Wistar rats. The protein fraction presenting such anti-inflammatory activity was purified and biochemically identified. The screening of the fraction responsible for such anti-inflammatory property was performed by evaluating the inhibition of carrageenan-induced paw edema in male Swiss mice. Biochemical analyses of the anti-inflammatory protein used chromatography, carbohydrates quantification of the protein sample, amino acids content analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Total sugar quantification revealed 32% glycosylation of the protein fraction. Amino acid analysis of such fraction showed a peculiar pattern presenting 29% valine. SDS-PAGE revealed that the protein sample is pure and its molecular weight is about 40kDa. Intravenous injection of the isolated substance into mice significantly inhibited carrageenan-induced paw edema. The isolated glycoprotein decreased carrageenan-induced paw edema in a prostaglandin-dependent phase, suggesting an inhibitory effect of the isolated glycoprotein on prostaglandin synthesis.
Palavras-chave
Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: J. Venom. Anim. Toxins incl. Trop. Dis. Ano de publicação: 2007 Tipo de documento: Article
Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: J. Venom. Anim. Toxins incl. Trop. Dis. Ano de publicação: 2007 Tipo de documento: Article