Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
Carnieli JR, P.; M. Ventura, A.; L. Durigon, E..
J. Venom. Anim. Toxins incl. Trop. Dis.;
13(4)2007.
Artigo
em Inglês
| VETINDEX
| ID: vti-443197
Resumo
A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.
Biblioteca responsável:
BR68.1
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