Time course of virus-like particles (VLPs) double-stranded rna accumulation in toxigenic and non-toxigenic strains of Aspergillus flavus
N. Silva, Valéria; Luiz Durigon, Edison; de Fátima Costa Pires, Maria; Lourenço, Alexandre; Jacinta de Faria, Maria; Corrêa, Benedito.
Braz. J. Microbiol.;
32(1)2001.
Artigo
em Inglês
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| ID: vti-443547
Resumo
Two strains of Aspergillus flavus, non-toxigenic NRRL 6550 and toxigenic NRRL 5940, were studied over a period of 44 days, in order to detect the presence of virus-like particles (VLPs) by means of electron microscopy (EM) and nucleic acids electrophoresis. Only the toxigenic strain contained VLPs, presenting three-segmented dsRNA. An increase in VLPs number was observed during the exponential phase of fungal growth, up to day 12 of culture; after this, higher levels of aflatoxin production in toxigenic NRRL 5940 mycelia occurred in parallel with decreased VLPs replication.Neste trabalho, foram estudadas duas cepas de Aspergillus flavus não toxigênica (NRRL 6550) e toxigênica (NRRL 5940). As cepas foram cultivadas durante 44 dias objetivando a pesquisa de partículas semelhantes a vírus (VLPs), por meio de microscopia eletrônica e pesquisa de ácidos nucléicos, através de eletroforese. Somente as cepas toxigênicas continham "VLPs", as quais apresentaram 3 segmentos de RNA de fita dupla. Um aumento no número de "VLPs" foi observado com 12 dias de cultivo, período correspondente a fase exponencial de crescimento de A. flavus.
Biblioteca responsável:
BR68.1
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